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Gene mapping in marsupials and monotremes

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To study the location of autosomal genes in the wallaroo (Macropits robustus), a number of rodent-kangaroo cell hybrids that retained wallaroo autosomes were typed for 24 enzymes. Expression of the kangaroo form of peptidase A (PEPA). mitochondrial malate dehydrogenase (MDH2). and nucleoside phos- phorylase (NP) was detected. Chromosome studies and enzyme analysis of hybrids, subclones, and revertants enabled confident assignment of PEPA to chromosome 4 and tentative assignment of MDH2 to chromosome I of this species.

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Cytogenetics and Cell Genetics

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