Detection of mouse natural killer T follicular helper (NKTFH) cells by flow cytometry

Chang, Pheh Ping; Vinuesa, Carola G.

Cultural advice

The Australian National University acknowledges, celebrates and pays our respects to the Ngunnawal and Ngambri people of the Canberra region and to all First Nations Australians on whose traditional lands we meet and work, and whose cultures are among the oldest continuing cultures in human history.

Aboriginal and Torres Strait Islander peoples are advised that ANU Library collections may include images, names, voices, and other representations of deceased persons.

Material in the collection may contain terms, language or views that reflect the period in which the item was created and may be considered inappropriate today.

Detection of mouse natural killer T follicular helper (NKTFH) cells by flow cytometry

Date

2015

Authors

Chang, Pheh Ping

Vinuesa, Carola G.

Abstract

Natural killer T follicular helper (NKTFH) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKTFH cells by multiparameter flow cytometer.