Bacterial Diversity Profiles of Desert Sand and Salt Crusts from the Gran Desierto de Altar, Sonora, Mexico

Abstract

The Gran Desierto de Altar, located in Sonora, Mexico, represents an arid region that offers an opportunity to study microbial life under extreme conditions. This study analyzed the bacterial diversity present in two distinct types of desert sediments: sand dunes (SDs) and salt crusts (SCs) by culture-dependent and culture-independent methods. Environmental DNA was obtained for 16S rRNA gene amplicon sequencing to obtain taxonomic information using QIIME2. In SD, the bacterial communities comprised 24 phyla, with Actinobacteriota (30–40%), Proteobacteria (22–27%), Bacteroidota (9–11%), Firmicutes (7–10%), and Chloroflexi (3–6%) emerging as the most abundant. Notably, in SC, the archaeal phylum Halobacterota was predominant (37–58%). SC hosted 12 bacterial phyla, the most abundant being Firmicutes (14–30%), Bacteroidota (3–24%), and Proteobacteria (1–12%). Bacteria belonging to the phylum Firmicutes, including Metabacillus niabensis, Bacillus subtilis, Bacillus licheniformis, and Alkalibacillus haloalkaliphilus, were isolated using nutrient and saline media. Overall, our findings indicate that the taxonomic composition of the samples analyzed from the Gran Desierto de Altar is consistent with that found in arid environments worldwide. This study provides a basis for future studies focusing on microbial diversity, genetic potentials, and resistance mechanisms of microorganisms from arid environments. Show more