Three-dimensional protein fold determination from backbone amide pseudocontact shifts generated by lanthanide tags at multiple sites
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Yagi, Hiromasa
Pilla, Kala
Maleckis, Ansis
Graham, Bim
Huber, Thomas
Otting, Gottfried
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Cell Press
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Site-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact shifts (PCS) in the nuclear magnetic resonance (NMR) spectra of the protein that are easily measured as changes in chemical shifts. By labeling the protein with
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2037-12-31
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