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A comparison of two procedures for labelling the surface of the hydatid disease organism, echinococcus granulosus, with 125I

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Date

1987

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Abstract

Living, intact protoscoleces of the British horse and sheep strains of Echinococcus granulosus were subjected to surface radioiodination procedures using 125I and Iodogen and 125I-Bolton Hunter reagent. Subsequent combined electron microscopy and autoradiography revealed specific surface membrane labelling with the Iodogen procedure, but significant tegumental labelling with the Bolton-Hunter reagent. The two parasite strains yielded different profiles of electrophoretically separated labelled proteins; the Iodogen method, not surprisingly, resulted in a less complex pattern of labelled polypeptides than the Bolton and Hunter reagent.

Description

Keywords

Echinococcus granulosus, hydatid, radiolabelling techniques, surface proteins

Citation

URI

https://dspace-test.anu.edu.au/handle/1885/733728188

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DevOps Pure Testing

Source

Journal of Helminthology

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Article

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DOI

10.1017/S0022149X00009718

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