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Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy

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Date

2017

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Abstract

Lattice light-sheet (LLS) microscopy provides ultrathin light sheets of a two-dimensional optical lattice that allows us imaging three-dimensional (3D) objects for hundreds of time points at sub-second intervals and at or below the diffraction limit. Galectin-3 (Gal3), a carbohydrate-binding protein, triggers glycosphingolipid (GSL)-dependent biogenesis of morphologically distinct endocytic vesicles that are cargo specific and clathrin independent. In this study, we apply LLS microscopy to study the dynamics of Gal3 dependent endocytosis in live T cells. This will allow us to observe Gal3-mediated endocytosis at high temporal and excellent 3D spatial resolution, which may shed light on our understanding of the mechanism and physiological function of Gal3-induced endocytosis.

Description

Keywords

3D imaging, endocytosis, galectin-3, Lattice light-sheet microscopy

Citation

URI

https://dspace-test.anu.edu.au/handle/1885/733737882

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DevOps Pure Testing

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Conference contribution

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DOI

10.1117/12.2275706

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