Walker, IanEaston, ChristopherOllis, David2015-12-132015-12-131359-7345http://hdl.handle.net/1885/90004Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase.Keywords: hydrolase; isomerase; lactone; article; catalysis; enzyme active site; enzyme activity; enzyme modification; hydrolysis; isomerism; site directed mutagenesis20002015-12-12