Chow, Michelle K.M.Amin, Abdullah A.Fulton, Kate F.Whisstock, James C.Buckle, Ashley M.Bottomley, Stephen P.2025-03-262025-03-26Scopus:32644489660PubMed:16150607https://dspace-test.anu.edu.au/handle/1885/733738874The expression and harvesting of proteins from insoluble inclusion bodies by solubilization and refolding is a technique commonly used in the production of recombinant proteins. To bring clarity to the large and widespread quantity of published protein refolding data, we have recently established the REFOLD database (http://refold.med.monash.edu.au), which is a freely available, open repository for protocols describing the refolding and purification of recombinant proteins. Refolding methods are currently published in many different formats and resources - REFOLD provides a standardized system for the structured reporting and presentation of these data. Furthermore, data in REFOLD are readily accessible using a simple search function, and the database also enables analyses which identify and highlight particular trends between suitable refolding and purification conditions and specific protein properties. This information may in turn serve to facilitate the rational design and development of new refolding protocols for novel proteins. There are approximately 200 proteins currently listed in REFOLD, and it is anticipated that with the continued contribution of data by researchers this number will grow significantly, thus strengthening the emerging trends and patterns and making this database a valuable tool for the scientific community.The authors acknowledge the contribution of all the researchers whose published data has been entered into REFOLD. This work was supported by grants from the National Health and Medical Research Council, the Victorian State Government, and the Victorian Partnership for Advanced Computing. SPB is a Monash University Senior Logan Fellow and NHMRC R.D. Wright Fellow. J.C.W. is a Monash University Logan Fellow and NHMRC Senior Research Fellow. K.F.F. is a NHMRC Peter Doherty Fellow.6EnglishDatabaseInclusion bodiesProtein refoldingRenaturation200610.1016/j.pep.2005.07.022http://www.scopus.com/inward/record.url?scp=32644489660&partnerID=8YFLogxK