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Protein determination on an automatic spectrophotometer

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Date

1982-03-15

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Abstract

Rapid and sensitive estimations of protein can be carried out in 96-well microplates in an automated spectrophotometer of the type developed for microenzyme linked immunosorbent assay determinations. The procedure is based on the Coomassie blue method of J. J. Sedmark and S. E. Grossberg (1977, Anal. Biochem. 79, 544-552) and can detect less than 200 ng of bovine plasma albumin and results in up to a 50-fold reduction in processing time over that required with a conventional manual spectrophotometer.

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Citation

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https://dspace-test.anu.edu.au/handle/1885/733724351

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DevOps Pure Testing

Source

Analytical Biochemistry

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Article

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DOI

10.1016/0003-2697(82)90578-4

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