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Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

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Date

2007

Authors

Wu, Peter
Ozawa, Kiyoshi
Lim, Siew P
Vasudevan, Subhash G.
Dixon, Nicholas Edward
Otting, Gottfried

Journal Title

Journal ISSN

Volume Title

Publisher

Wiley-VCH Verlag GMBH

Abstract

Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).

Description

Keywords

Keywords: Cell culture; Cyclization; Enzyme kinetics; Proteins; Reaction kinetics; Transcription; Cell-free protein synthesis; High-throughput methods; Polymerase chain reaction; DNA; cell extract; DNA; article; cell free system; cytology; Escherichia coli; genetic Cell-free protein synthesis; High-throughput methods; NMR spectroscopy; Polymerase chain reaction

Citation

URI

http://hdl.handle.net/1885/28139

Collections

Open Access Research

Source

Angewandte Chemie International Edition

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1002/anie.200605237

Restricted until

2037-12-31

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File
Description
01_Wu_Cell-free_2007.pdf (174.59 KB)

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