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Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing

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Authors

Clermont, Olivier
Christenson, Julia
Daubie, Anne-Sophie
Gordon, David
Denamur, Erick

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Publisher

Elsevier

Abstract

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

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Source

Journal of Microbiological Methods

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Restricted until

2037-12-31