Localization of legumin and vicilin in bean cotyledon cells using fluorescent antibodies

Abstract

SUBCELLULAR fractions of bulked protein bodies isolated from the storage tissues of seeds contain several or many types of protein1-3, but in general it is not known whether this diversity occurs within an individual protein body, or whether the different proteins are deposited in separate compartments during seed maturation. Immunofluorescence techniques have enabled us to resolve this question in respect of the subcellular distribution of legumin and vicilin, two important globulins stored in the cotyledons of many legumes4-6. Show more