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Identification of the Echinococcus (hydatid disease) organisms using cloned DNA markers

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Date

1985

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Abstract

Cloned DNA fragments of the ribosomal RNA gene of Schistosoma mansoni hybridise strongly to Echinococcus DNA following restriction endonuclease and Southern transfer analysis. Individuals within a strain of E. granulosus exhibit identical patterns of hybridisation. However, the hybridisation patterns show significant differences between E. granulosus and E. multilocularis, and between the horse and sheep strains of E. granulosus. This technique represents a powerful, additional method for the identification and characterisation of new isolates of E. granulosus and E. multilocularis.

Description

Keywords

Cloned DNA, Echinococcus granulosus, Echinococcus multilocularis, Hydatid disease, Restriction endonuclease, Ribosomal RNA genes, Southern transfer analysis, Strain characterization

Citation

URI

https://dspace-test.anu.edu.au/handle/1885/733739278

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DevOps Pure Testing

Source

Molecular and Biochemical Parasitology

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Article

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DOI

10.1016/0166-6851(85)90016-7

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