Assignment of cloned genes to electrophoretically separated chromosomes of the yeast Pachysolen tannophilus
Abstract
An electrophoretic karyotype of Pachysolen tannophilus has been obtained using Pulsed Field Gel Electrophoresis. Seven chromosomal bands were separated with one of the bands migrating probably as a doublet. The sizes of the chromosomes were estimated to be between 1 and 3.1 megabase pairs. Eleven loci have been assigned to chromosomal bands, including four involved in the metabolism of df-xylose.
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FEMS Microbiology Letters